Coding
Part:BBa_K2984031
Designed by: Dimitri Schumacher Group: iGEM19_Humboldt_Berlin (2019-10-16)
L1c-PsaD-ARS-PETase-YFP-RBCS2
This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the ARS secretion signal, the PETase enzyme, a YFP mVenus marker, and the Rbcs2 terminator. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1979
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1308
Illegal PstI site found at 1979 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal XhoI site found at 1032 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1979
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1979
Illegal NgoMIV site found at 2456 - 1000COMPATIBLE WITH RFC[1000]
Characterization
[edit]
Categories
Parameters
chassis | C. reinhardtii |
emission | 528 nm |
excitation | 515 nm |
protein | PETase-YFP |